A novel blood-feeding detoxification pathway in Nippostrongylus brasiliensis L3 reveals a potential checkpoint for arresting hookworm development
Tiffany Bouchery, Kara Filbey, Amy Shepherd, Jodie Chandler, Deepa Patel, Alfonso Schmidt, Mali Camberis, Adeline Peignier, Adam AT Smith, Karen Johnston, Gavin Painter, Mark Pearson, Paul Giacomin, Alex Loukas, Maria-Elena Bottazzi, Peter Hotez, Graham LeGros
PLoS Pathogens | PUBLIC LIBRARY SCIENCE | Published : 2018
As part of on-going efforts to control hookworm infection, the "human hookworm vaccine initiative" has recognised blood feeding as a feasible therapeutic target for inducing immunity against hookworm infection. To this end, molecular approaches have been used to identify candidate targets, such as Necator americanus (Na) haemoglobinase aspartic protease-1 (APR-1), with immunogenicity profiled in canine and hamster models. We sought to accelerate the immune analysis of these identified therapeutic targets by developing an appropriate mouse model. Here we demonstrate that Nippostrongylus brasiliensis (Nb), a phylogenetically distant strongylid nematode of rodents, begins blood feeding early in..View full abstract
This work has been supported by the Marjorie Barclay Trust and the Health Research Council of New Zealand (http://www.hrc.govt.nz). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.