Urine Proteome Analysis Reflects Atherosclerotic Disease in an ApoE(-/-) Mouse Model and Allows the Discovery of New Candidate Biomarkers in Mouse and Human Atherosclerosis
Constantin von zur Muhlen, Eric Schiffer, Christine Sackmann, Petra Zuerbig, Irene Neudorfer, Andreas Zirlik, Nay Htun, Alexander Iphoefer, Lothar Jaensch, Harald Mischak, Christoph Bode, Yung C Chen, Karlheinz Peter
Molecular & Cellular Proteomics | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | Published : 2012
Noninvasive diagnosis of atherosclerosis via single biomarkers has been attempted but remains elusive. However, a previous polymarker or pattern approach of urine polypeptides in humans reflected coronary artery disease with high accuracy. The aim of the current study is to use urine proteomics in ApoE(-/-) mice to discover proteins with pathophysiological roles in atherogenesis and to identify urinary polypeptide patterns reflecting early stages of atherosclerosis. Urine of ApoE(-/-) mice either on high fat diet (HFD) or chow diet was collected over 12 weeks; urine of wild type mice on HFD was used to exclude diet-related proteome changes. Capillary electrophoresis coupled to mass spectrome..View full abstract
Awarded by Deutsche Forschungsgemeinschaft
Awarded by European Union
This work was supported in part by Deutsche Forschungsgemeinschaft Grant MU2727/3-1 (to C. v. z. M.), European Union Sixth Framework Programme InGenious HyperCare Grant LSHM-CT-2006-037093, SysKID Grant HEALTH-F2-2009-241544 (to E. S., P. Z., and H. M.), and a Future Fellowship from the Australian Research Council (to K. P.).