Journal article
Urine proteome analysis reflects atherosclerotic disease in an ApoE -/- mouse model and allows the discovery of new candidate biomarkers in mouse and human atherosclerosis
C Von Zur Muhlen, E Schiffer, C Sackmann, P Zürbig, I Neudorfer, A Zirlik, N Htun, A Ipho, L Jänsch, H Mischak, C Bode, YC Chen, K Peter
Molecular and Cellular Proteomics | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | Published : 2012
Abstract
Noninvasive diagnosis of atherosclerosis via single biomarkers has been attempted but remains elusive. However, a previous polymarker or pattern approach of urine polypeptides in humans reflected coronary artery disease with high accuracy. The aim of the current study is to use urine proteomics in ApoE-/- mice to discover proteins with pathophysiological roles in atherogenesis and to identify urinary polypeptide patterns reflecting early stages of atherosclerosis. Urine of ApoE-/- mice either on high fat diet (HFD) or chow diet was collected over 12 weeks; urine of wild type mice on HFD was used to exclude diet-related proteome changes. Capillary electrophoresis coupled to mass spectrometry ..
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Awarded by European Commission
Funding Acknowledgements
This work was supported in part by Deutsche Forschungsgemeinschaft Grant MU2727/3-1 (to C. v. z. M.), European Union Sixth Framework Programme InGenious HyperCare Grant LSHM-CT-2006-037093, SysKID Grant HEALTH-F2-2009-241544 (to E. S., P. Z., and H. M.), and a Future Fellowship from the Australian Research Council (to K. P.).