Multiharmonic-generation biopsy of skin.
Chi-Kuang Sun, Cheng-Chi Chen, Shi-Wei Chu, Tsung-Han Tsai, Yung-Chih Chen, Bai-Ling Lin
Optics Letters | Published : 2003
Because it avoids the in-focus photodamage and phototoxicity problem of two-photon-fluorescence excitation, multiharmonic-generation biopsy based on a 1200-1300-nm light source could provide a truly noninvasive and highly penetrative optical sectioning of skin. We study multiharmonic-generation biopsy of fixed mouse skin. Our preliminary study suggests that this technique could provide submicrometer-resolution deep-tissue noninvasive biopsy images in skin without the use of fluorescence and exogenous markers.