Evaluation of Urine Proteome Pattern Analysis for Its Potential To Reflect Coronary Artery Atherosclerosis in Symptomatic Patients
Constantin von zur Muhlen, Eric Schiffer, Petra Zuerbig, Marcus Kellmann, Mario Brasse, Natalie Meert, Raymond C Vanholder, Anna F Dominiczak, Yung Chih Chen, Harald Mischak, Christoph Bode, Karlheinz Peter
Journal of Proteome Research | AMER CHEMICAL SOC | Published : 2009
Coronary artery disease (CAD) is a major cause of mortality and morbidity. Noninvasive proteome analysis could guide clinical evaluation and early/preventive treatment. Under routine clinical conditions, urine of 67 patients presenting with symptoms suspicious for CAD were analyzed by capillary electrophoresis directly coupled with mass spectrometry (CE-MS). All patients were subjected to coronary angiography and either assigned to a CAD or non-CAD group. A training set of 29 patients was used to establish CAD and non-CAD-associated proteome patterns of plasma as well as urine. Significant discriminatory power was achieved in urine but not in plasma. Therefore, urine proteomic analysis of fu..View full abstract
Awarded by EU InGenious HyperCare
We are grateful to Dr. Sheng Chen for technical assistance, to Dr. Belinda Smith for critical reading of the manuscript, and to Prof. Alex Bobik for providing human atherosclerotic plaque samples and advice in histological preparations. This work was funded by EU InGenious HyperCare LSHM-CT-2006-037093 to E.S., P.Z., A.F.D., and H.M. and the National Heart Foundation of Australia.