P773 Post-operative Crohn's disease recurrence is associated with specific changes in the faecal microbiome – potential pathogenic and protective roles

Abstract

Background: Crohn's disease usually recurs after “curative” resection. This may relate to specific microbial populations playing a pathogenic role. Methods: Faecal samples were obtained peri-operatively (baseline) and at 6, 12 and 18 months after surgery from 130 patients enrolled in the prospective POCER study. Endoscopic disease recurrence was assessed (Rutgeerts Score ≥i2) at six months (in 2/3 of patients in the active endoscopic care arm) and at 18 months in all patients. DNA was extracted using the MoBio Powersoil DNA extraction kit, and the V2 region of the bacterial 16S rRNA gene sequenced (MiSeq). Data were processed using the QIIME pipeline; alpha and beta diversity were assessed o..