Single-Cell Flow Cytometry Profiling of BAL in Children
Shivanthan Shanthikumar, Matthew Burton, Richard Saffery, Sarath C Ranganathan, Melanie R Neeland
American Journal of Respiratory Cell and Molecular Biology | American Thoracic Society | Published : 2020
Childhood pulmonary diseases not only cause childhood morbidity and mortality but also can cause long-term pulmonary impairment. The clinical management of many childhood pulmonary diseases is hampered by a limited understanding of the underlying pathophysiological mechanisms. Flow cytometry, which can be used to phenotype individual cell populations or isolate cells for downstream analysis, represents a crucial technology that can help to elucidate the pathophysiology of these conditions. Here, we describe a flow cytometry–based method for purification and characterization of cell populations in BAL from children. This includes assessment of the effect of cryopreservation on cell phenotype ..View full abstract
Supported by a Vertex Innovation Award by Vertex Pharmaceuticals and a Melbourne Children's LifeCourse Postdoctoral Fellowship (M.R.N.). Vertex did not have any input into study design, study execution, analysis of results or manuscript development.