A method for gene knockdown in the retina using a lipid-based carrier
Joshua A Chu-Tan, Nilisha Fernando, Riemke Aggio-Bruce, Adrian Cioanca, Krisztina Valter, Nektaria Andronikou, Xavier deMollerat du Jeu, Matt Rutar, Jan Provis, Riccardo Natoli
MOLECULAR VISION | MOLECULAR VISION | Published : 2020
Purpose: The use of small non-coding nucleic acids, such as siRNA and miRNA, has allowed for a deeper understanding of gene functions, as well as for development of gene therapies for complex neurodegenerative diseases, including retinal degeneration. For effective delivery into the eye and transfection of the retina, suitable transfection methods are required. We investigated the use of a lipid-based transfection agent, Invivofectamine® 3.0 (Thermo Fisher Scientific), as a potential method for delivery of nucleic acids to the retina. Methods: Rodents were injected intravitreally with formulations of Invivofectamine 3.0 containing scrambled, Gapdh, Il-1β, and C3 siRNAs, or sterile PBS (contr..View full abstract
Awarded by National Health and Medical Research Council
This study was partly supported through funding from the Thermo Fisher Scientific Applications and Claims Expansions Grant with two of the authors (NA and XM) members of the Thermo Fisher Scientific team. Other funding bodies are The National Health and Medical Research Council (APP1127705, 2017-2019), the Australian Government Research Training Program Scholarship and the Australian National University Translational Fellowship. The authors do not have any other competing interests or conflicts of interest to disclose with regards to this paper.