High-Resolution Confocal Fluorescence Imaging of Serine Hydrolase Activity in Cryosections - Application to Glioma Brain Unveils Activity Hotspots Originating from Tumor-Associated Neutrophils
Niina Aaltonen, Prosanta K Singha, Hermina Jakupovic, Thomas Wirth, Haritha Samaranayake, Sanna Pasonen-Seppanen, Kirsi Rilla, Markku Varjosalo, Laura E Edgington-Mitchell, Paulina Kasperkiewicz, Marcin Drag, Sara Kalvala, Eemeli Moisio, Juha R Savinainen, Jarmo T Laitinen
Biological Procedures Online | BMC | Published : 2020
Background: Serine hydrolases (SHs) are a functionally diverse family of enzymes playing pivotal roles in health and disease and have emerged as important therapeutic targets in many clinical conditions. Activity-based protein profiling (ABPP) using fluorophosphonate (FP) probes has been a powerful chemoproteomic approach in studies unveiling roles of SHs in various biological systems. ABPP utilizes cell/tissue proteomes and features the FP-warhead, linked to a fluorescent reporter for in-gel fluorescence imaging or a biotin tag for streptavidin enrichment and LC-MS/MS-based target identification. Existing ABPP approaches characterize global SH activity based on mobility in gel or MS-based t..View full abstract
Awarded by Finnish Academy
Awarded by Australian Research Council (ARC)
J.T.L. was supported by the Finnish Academy (grant no. 278212). L.E.M. was supported by a Grimwade Fellowship from the Russell and Mab Grimwade Miegunyah Fund at The University of Melbourne and a DECRA Fellowship from the Australian Research Council (ARC, DE180100418). Drag laboratory is supported by Foundation for Polish Science and National Science Centre in Poland. PK. is the beneficiary of a L'Oreal Poland and the Polish' Ministry of Science and Higher Education scholarships.