Journal article

Effects of testosterone suppression, hindlimb immobilization, and recovery on [H-3]ouabain binding site content and Na , K -ATPase isoforms in rat soleus muscle

Muath M Altarawneh, Erik D Hanson, Andrew C Betik, Aaron C Petersen, Alan Hayes, Michael J McKenna

Journal of Applied Physiology | AMER PHYSIOLOGICAL SOC | Published : 2020

Abstract

We investigated the effects of testosterone suppression, hindlimb immobilization, and recovery on skeletal muscle Na+,K+-ATPase (NKA), measured via [3H]ouabain binding site content (OB) and NKA isoform abundances (α1-3, β1-2). Male rats underwent castration or sham surgery plus 7 days of rest, 10 days of unilateral immobilization (cast), and 14 days of recovery, with soleus muscles obtained at each time from cast and noncast legs. Testosterone reduction did not modify OB or NKA isoforms in nonimmobilized control muscles. With sham surgery, OB was lower after immobilization in the cast leg than in both the noncast leg (-26%, P = 0.023) and the nonimmobilized control (-34%, P = 0.001), but OB ..

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University of Melbourne Researchers