Journal article

Structural basis of substrate recognition and catalysis by fucosyltransferase 8

Michael A Jarva, Marija Dramicanin, James P Lingford, Runyu Mao, Alan John, Kate E Jarman, Rhys Grinter, Ethan D Goddard-Borger

Journal of Biological Chemistry | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | Published : 2020

Abstract

Fucosylation of the innermost GlcNAc of N-glycans by fucosyltransferase 8 (FUT8) is an important step in the maturation of complex and hybrid N-glycans. This simple modification can dramatically affect the activities and half-lives of glycoproteins, effects that are relevant to understanding the invasiveness of some cancers, development of mAb therapeutics, and the etiology of a congenital glycosylation disorder. The acceptor substrate preferences of FUT8 are well-characterized and provide a framework for understanding N-glycan maturation in the Golgi; however, the structural basis of these substrate preferences and the mechanism through which catalysis is achieved remain unknown. Here we de..

View full abstract

Grants

Awarded by National Health and Medical Research Council of Australia


Funding Acknowledgements

This work was supported by The Walter and Eliza Hall Institute of Medical Research, National Health and Medical Research Council of Australia Project Grant GNT1139549, the Australian Cancer Research Fund, and a Victorian State Government Operational Infrastructure support grant. The authors declare that they have no conflicts of interest with the contents of this article.