Journal article

Disruption of Phosphate Homeostasis Sensitizes Staphylococcus aureus to Nutritional Immunity

Jessica L Kelliher, Erin B Brazel, Jana N Radin, Eliot S Joya, Paola K Parraga Solorzano, Stephanie L Neville, Christopher A McDevitt, Thomas E Kehl-Fie



To control infection, mammals actively withhold essential nutrients, including the transition metal manganese, by a process termed nutritional immunity. A critical component of this host response is the manganese-chelating protein calprotectin. While many bacterial mechanisms for overcoming nutritional immunity have been identified, the intersection between metal starvation and other essential inorganic nutrients has not been investigated. Here, we report that overexpression of an operon encoding a highly conserved inorganic phosphate importer, PstSCAB, increases the sensitivity of Staphylococcus aureus to calprotectin-mediated manganese sequestration. Further analysis revealed that overexpr..

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Awarded by National Institutes of Health

Awarded by National Health and Medical Research Council (NHMRC)

Awarded by Australian Research Council (ARC)

Funding Acknowledgements

This work was supported by the Vallee Foundation, by a Basil O'Connor award from the March of Dimes, and by National Institutes of Health grants K22 AI104805, R01 AI118880, and R21 AI149115 to T.E.K.-F. and in part by a James R. Beck Graduate Research Fellowship in Microbiology awarded to J.L.K. Work in the McDevitt laboratory was supported by National Health and Medical Research Council (NHMRC) project grants 1122582 and 1140554 and by an Australian Research Council (ARC) Discovery Project grant to C.A.M. (DP170102102). S.L.N. is an NHMRC Early Career Research Fellow (1142695), and C.A.M. is an ARC Future Fellow (FT170100006). The funding agencies had no role in study design, data collection and interpretation, or the decision to submit the work for publication.