Journal article

Voltage-Sensor Transitions of the Inward-Rectifying K Channel KAT1 Indicate a Latching Mechanism Biased by Hydration within the Voltage Sensor

Cecile Lefoulon, Rucha Karnik, Annegret Honsbein, Paul Vijay Gutla, Christopher Grefen, Janin Riedelsberger, Tomas Poblete, Ingo Dreyer, Wendy Gonzalez, Michael R Blatt

Plant Physiology | AMER SOC PLANT BIOLOGISTS | Published : 2014

Abstract

The Kv-like (potassium voltage-dependent) K(+) channels at the plasma membrane, including the inward-rectifying KAT1 K(+) channel of Arabidopsis (Arabidopsis thaliana), are important targets for manipulating K(+) homeostasis in plants. Gating modification, especially, has been identified as a promising means by which to engineer plants with improved characteristics in mineral and water use. Understanding plant K(+) channel gating poses several challenges, despite many similarities to that of mammalian Kv and Shaker channel models. We have used site-directed mutagenesis to explore residues that are thought to form two electrostatic countercharge centers on either side of a conserved phenylala..

View full abstract

Grants

Awarded by Biotechnology and Biological Sciences Research Council


Awarded by ANILLO


Awarded by Deutsche Forschungsgemeinschaft


Funding Acknowledgements

This work was supported by the Biotechnology and Biological Sciences Research Council (grant nos. BB/H001673/1, BB/H024867/1, BB/H009817/1, and BB/K015893/1 to M.R.B.), by ANILLO (grant no. ACT1104 to W.G. and J.R.), and by the Deutsche Forschungsgemeinschaft (grant no. DR430/8-1 to I.D., W.G., and J.R.).