Journal article

Sustained subcutaneous delivery of secretome of human cardiac stem cells promotes cardiac repair following myocardial infarction

Andrew R Kompa, David W Greening, Anne M Kong, Paul J McMillan, Haoyun Fang, Ritika Saxena, Raymond CB Wong, Jarmon G Lees, Priyadharshini Sivakumaran, Andrew E Newcomb, Bakhos A Tannous, Cameron Kos, Lina Mariana, Thomas Loudovaris, Derek J Hausenloy, Shiang Y Lim

Cardiovascular Research | OXFORD UNIV PRESS | Published : 2021

Abstract

AIMS: To establish pre-clinical proof of concept that sustained subcutaneous delivery of the secretome of human cardiac stem cells (CSCs) can be achieved in vivo to produce significant cardioreparative outcomes in the setting of myocardial infarction. METHODS AND RESULTS: Rats were subjected to permanent ligation of left anterior descending coronary artery and randomized to receive subcutaneous implantation of TheraCyte devices containing either culture media as control or 1 × 106 human W8B2+ CSCs, immediately following myocardial ischaemia. At 4 weeks following myocardial infarction, rats treated with W8B2+ CSCs encapsulated within the TheraCyte device showed preserved left ventricular ejec..

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Grants

Awarded by British Heart Foundation


Awarded by Singapore Ministry of Health's National Medical Research Council


Awarded by Singapore Ministry of Education Academic Research Fund Tier 2


Awarded by National Health and Medical Research Council


Awarded by COST (European Cooperation in Science and Technology)


Funding Acknowledgements

This work was performed with support from the St Vincent's Hospital (Melbourne) Research Endowment Fund and Stafford Fox Medical Research Foundation. Derek Hausenloy was supported by the British Heart Foundation (CS/14/3/31002); the National Institute for Health Research University College London Hospitals Biomedical Research Centre; Duke-National University Singapore Medical School; Singapore Ministry of Health's National Medical Research Council under its Clinician Scientist-Senior Investigator scheme (NMRC/CSA-SI/0011/2017) and Collaborative Centre Grant scheme (NMRC/CGAug16C006); and the Singapore Ministry of Education Academic Research Fund Tier 2 (MOE2016-T2-2-021). This work was also funded in part by National Health and Medical Research Council project grants (#1057741 and #1139489 to D.W.G.), and Helen Amelia Hanis Fellowship (to D.W.G.). The O'Brien Institute Department of St Vincent's Institute of Medical Research and the Centre for Eye Research Australia receive Operational Infrastructure Support from the Victorian State Government's Department of Innovation, Industry and Regional Development. We thank Charles P. Lai and Bakhos A. Tannous for providing the CSCW-GlucB-IRES-GFP and CSCW-sshBirA-IRESmCherry plasmids, and Layal Wazen for the generation of the lentiviruses. This article is based upon work from COST Action EUCARDIOPROTECTION CA16225 supported by COST (European Cooperation in Science and Technology).