A single whole-body low dose X-irradiation does not affect L1, B1 and IAP repeat element DNA methylation longitudinally
Michelle R Newman, Pamela J Sykes, Benjamin J Blyth, Eva Bezak, Mark D Lawrence, Katherine L Morel, Rebecca J Ormsby
PLoS One | PLOS | Published : 2014
The low dose radioadaptive response has been shown to be protective against high doses of radiation as well as aging-induced genomic instability. We hypothesised that a single whole-body exposure of low dose radiation would induce a radioadaptive response thereby reducing or abrogating aging-related changes in repeat element DNA methylation in mice. Following sham or 10 mGy X-irradiation, serial peripheral blood sampling was performed and differences in Long Interspersed Nucleic Element 1 (L1), B1 and Intracisternal-A-Particle (IAP) repeat element methylation between samples were assessed using high resolution melt analysis of PCR amplicons. By 420 days post-irradiation, neither radiation- o..View full abstract
Awarded by Low Dose Radiation Research Program, Biological and Environmental Research, U.S. Department of Energy
This work was supported by the Low Dose Radiation Research Program, Biological and Environmental Research, U.S. Department of Energy (DE-FG02-05ER64104); the Flinders Medical Centre/Flinders Centre for Innovation in Cancer Research Grant; and the Flinders Medical Centre Foundation and BHP Billiton Low Dose Radiation Research Scholarship (to M.N.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.