A Host-Specific Blocking Primer Combined with Optimal DNA Extraction Improves the Detection Capability of a Metabarcoding Protocol for Canine Vector-Borne Bacteria
Lucas G Huggins, Anson Koehler, Bettina Schunack, Tawin Inpankaew, Rebecca J Traub
PATHOGENS | MDPI | Published : 2020
Bacterial canine vector-borne diseases are responsible for some of the most life-threatening conditions of dogs in the tropics and are typically poorly researched with some presenting a zoonotic risk to cohabiting people. Next-generation sequencing based methodologies have been demonstrated to accurately characterise a diverse range of vector-borne bacteria in dogs, whilst also proving to be more sensitive than conventional PCR techniques. We report two improvements to a previously developed metabarcoding tool that increased the sensitivity and diversity of vector-borne bacteria detected from canine blood. Firstly, we developed and tested a canine-specific blocking primer that prevents cross..View full abstract
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Awarded by Australian Research Council
This research was funded by an Australian Research Council Linkage grant LP170100187 with Bayer Animal Health GmbH and Bayer Australia as industry partners. Financial support was also provided by the University of Melbourne postgraduate scholarship scheme.