Journal article

Distribution of Parkinson's disease associated RAB39B in mouse brain tissue

Yujing Gao, Gabrielle R Wilson, Sarah EM Stephenson, Mustapha Oulad-Abdelghani, Nicolas Charlet-Berguerand, Kiymet Bozaoglu, Catriona A McLean, Paul Q Thomas, David Finkelstein, Paul Lockhart

MOLECULAR BRAIN | BMC | Published : 2020

Abstract

Pathogenic variants in the gene encoding the small GTPase Ras analogue in Brain 39b (RAB39B) are associated with early-onset parkinsonism. In this study we investigated the expression and localization of RAB39B (RNA and protein) in mouse brain tissue to gain a better understanding of its normal physiological function(s) and role in disease.We developed novel resources, including monoclonal antibodies directed against RAB39B and mice with Rab39b knockout, and performed real-time PCR and western blot analysis on whole brain lysates. To determine the spatial localization of Rab39b RNA and protein, we performed in-situ hybridization and immunohistochemistry on fresh frozen and fixed brain tissue..

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Grants

Awarded by National Health & Medical Research Council (Australia)


Awarded by Michael J Fox Foundation/Shake It Up


Funding Acknowledgements

This work was funded in part by the McCusker Charitable Foundation, National Health & Medical Research Council (Australia) project grants (GNT1041860, GNT1144724) and the Michael J Fox Foundation/Shake It Up Grant 12173 to PJL. Additional infrastructure funding to the Murdoch Children's Research Institute was provided by the Australian Government National Health and Medical Research Council Independent Research Institute Infrastructure Support Scheme and the Victorian Government's Operational Infrastructure Support Program. PJL is supported by the Vincent Chiodo Foundation. The funding bodies had no role in the design, analysis, interpretation or reporting of results.