Journal article

Identification and characterization of an R-Smad homologue (Hco-DAF-8) from Haemonchus contortus

Fang-Fang Li, Robin B Gasser, Feng Liu, Jia-Nan Shan, Wen-Da Di, Li He, Cai-Xian Zhou, Chun-Qun Wang, Rui Fang, Min Hu

PARASITES & VECTORS | BMC | Published : 2020

Abstract

BACKGROUND: Smad proteins are essential cellular mediators within the transforming growth factor-β (TGF-β) superfamily. They directly transmit incoming signals from the cell surface receptors to the nucleus. In spite of their functional importance, almost nothing is known about Smad proteins in parasitic nematodes including Haemonchus contortus, an important blood-sucking nematode of small ruminants. METHODS: Based on genomic and transcriptome data for H. contortus and using bioinformatics methods, a Smad homologue (called Hco-daf-8) was inferred from H. contortus and the structural characteristics of this gene and its encoded protein Hco-DAF-8 established. Using real-time PCR and immunofluo..

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Grants

Awarded by National Key Basic Research Program (973 program) of China


Awarded by National Natural Science Foundation


Awarded by Huazhong Agricultural University Scientific & Technological Self Innovation Foundation


Awarded by Natural Science Foundation of Hubei Province


Funding Acknowledgements

This study was supported by the National Key Basic Research Program (973 program) of China (grant no. 2015CB150300) and the National Natural Science Foundation (grant no. 31872462; MH); the Huazhong Agricultural University Scientific & Technological Self Innovation Foundation (program no. 2015RC005) and the Australian Research Council (ARC; RBG) and the Natural Science Foundation of Hubei Province (grant no. 2017CFA020; MH and FR).