Platelets disrupt vasculogenic mimicry by cancer cells
Carmela Martini, Emma J Thompson, Stephanie R Hyslop, Michaelia P Cockshell, Brian J Dale, Lisa M Ebert, Anthony E Woods, Emma C Josefsson, Claudine S Bonder
Scientific Reports | NATURE PUBLISHING GROUP | Published : 2020
Tumour vasculature supports the growth and progression of solid cancers with both angiogenesis (endothelial cell proliferation) and vasculogenic mimicry (VM, the formation of vascular structures by cancer cells themselves) predictors of poor patient outcomes. Increased circulating platelet counts also predict poor outcome for cancer patients but the influence of platelets on tumour vasculature is incompletely understood. Herein, we show with in vitro assays that platelets did not influence angiogenesis but did actively inhibit VM formation by cancer cell lines. Both platelet sized beads and the releasates from platelets were partially effective at inhibiting VM formation suggesting that dire..View full abstract
Awarded by NHMRC
Awarded by Cancer Australia & the National Breast Cancer Foundation
Awarded by Independent Research Institutes Infrastructure Support Scheme
The authors thank Samantha Escarbe and Jason Corban for expert technical assistance, Prof. Benjamin Kile for the Bcl-xPlt20/Plt20 mice, as well as blood donors and researchers within the Centre for Cancer Biology and University of South Australia. This project was supported by grants to C.S.B. from the NHMRC [GNT1022150], Cancer Australia & the National Breast Cancer Foundation , the Royal Adelaide Hospital Research Foundation; funding to E.C.J. from Independent Research Institutes Infrastructure Support Scheme (9000220), a Victorian State Government Operational Infrastructure Support Grant and a fellowship from the Lorenzo and Pamela Galli Charitable Trust; as well as Australian Postgraduate Awards to C.M., E.J.T. and S.R.H.