Quantitative Phase Imaging Using Digital Holographic Microscopy Reliably Assesses Morphology and Reflects Elastic Properties of Fibrotic Intestinal Tissue
Arne Bokemeyer, Phil Robin Tepasse, Lena Quill, Philipp Lenz, Emile Rijcken, Michael Vieth, Nik Ding, Steffi Ketelhut, Florian Rieder, Bjoern Kemper, Dominik Bettenworth
Scientific Reports | NATURE PUBLISHING GROUP | Published : 2019
Intestinal strictures are a frequent complication in patients with Crohn's Disease (CD) and the presence of fibrosis within strictures impacts the therapeutic treatment approach. Here, we evaluate quantitative phase imaging (QPI) using digital holographic microscopy (DHM) for the evaluation of fibrosis within CD strictures. 30 full thickness resection specimens were obtained from non-stenotic and stenotic tissue areas of 15 CD patients. Cryostat sections were analyzed by DHM to measure the spatial distribution of the refractive index (RI) to quantify tissue density. Complementary, histopathological evaluation of H&E staining and immunofluorescence (IF) targeting fibrosis markers served as th..View full abstract
We thank Sonja Dufentester and Elke Weber for expert technical assistance. We thank Optics11 (Amsterdam, N.L.) for providing the timely restricted use of a cantilever-based nanointender (Piuma Nanoindenter). We acknowledge support by Open Access Publication Fund of the University of Muenster. We thank Willi Kramer for helping to illustrate Fig. 1A.