Implementing Disposables Technology, Delivering Innovation, and Transforming an Organization

Abstract

As part of the Sanofi Pasteur Knowledge & Innovat ion for Technology Excel lence (KITE) initiative, we have been able to demonstrate the feasibi l ity of large-scale Vero cell culture in disposable bioreactors up to the 500-L scale. This included successful integration of the disposable process from seed to large-scale cell expansion and infection, including cell thawing and expansion directly in the disposable Nucleo 20 (a closed system for sampling, decontamination, and waste elimination) up to a 500-L working volume. Good virus yield and productivity were obtained in 20-, 200-, and 500-L scale disposable bioreactors. By using a validated BioSolve process modeling platform, we were able to..