Proteolytic dynamics of human 20S thymoproteasome
Ulrike Kuckelkorn, Sabine Stuebler, Kathrin Textoris-Taube, Christiane Kilian, Agathe Niewienda, Petra Henklein, Katharina Janek, Michael PH Stumpf, Michele Mishto, Juliane Liepe
JOURNAL OF BIOLOGICAL CHEMISTRY | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | Published : 2019
An efficient immunosurveillance of CD8+ T cells in the periphery depends on positive/negative selection of thymocytes and thus on the dynamics of antigen degradation and epitope production by thymoproteasome and immunoproteasome in the thymus. Although studies in mouse systems have shown how thymoproteasome activity differs from that of immunoproteasome and strongly impacts the T cell repertoire, the proteolytic dynamics and the regulation of human thymoproteasome are unknown. By combining biochemical and computational modeling approaches, we show here that human 20S thymoproteasome and immunoproteasome differ not only in the proteolytic activity of the catalytic sites but also in the peptid..View full abstract
Awarded by Berlin Institute of Health
This work was supported in part by Cancer Research UK King's Health Partners Centre at King's College London (Development Fund 2018) (to M. M.), Berlin Institute of Health Grant CRG1-TP1 (to P. M. Kloetzel). The authors declare that they have no conflicts of interest with the contents of this article.