Journal article

Proteolytic dynamics of human 20S thymoproteasome

Ulrike Kuckelkorn, Sabine Stuebler, Kathrin Textoris-Taube, Christiane Kilian, Agathe Niewienda, Petra Henklein, Katharina Janek, Michael PH Stumpf, Michele Mishto, Juliane Liepe

JOURNAL OF BIOLOGICAL CHEMISTRY | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | Published : 2019

Abstract

An efficient immunosurveillance of CD8+ T cells in the periphery depends on positive/negative selection of thymocytes and thus on the dynamics of antigen degradation and epitope production by thymoproteasome and immunoproteasome in the thymus. Although studies in mouse systems have shown how thymoproteasome activity differs from that of immunoproteasome and strongly impacts the T cell repertoire, the proteolytic dynamics and the regulation of human thymoproteasome are unknown. By combining biochemical and computational modeling approaches, we show here that human 20S thymoproteasome and immunoproteasome differ not only in the proteolytic activity of the catalytic sites but also in the peptid..

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University of Melbourne Researchers