Journal article

Origins of cell-to-cell variability, kinetic proof-reading and the robustness of MAPK signal transduction

Sarah Filippi, Chris Barnes, Paul Kirk, Takamasa Kudo, Siobhan McMahon, Takaho Tsuchiya, Takumi Wada, Shinya Kuroda, Michael Stumpf

Published : 2015

Abstract

Cellular signalling processes can exhibit pronounced cell-to-cell variability in genetically identical cells. This affects how individual cells respond differentially to the same environmental stimulus. However, the origins of cell-to-cell variability in cellular signalling systems remain poorly understood. Here we measure the temporal evolution of phosphorylated MEK and ERK dynamics across populations of cells and quantify the levels of population heterogeneity over time using high-throughput image cytometry. We use a statistical modelling framework to show that upstream noise is the dominant factor causing cell-to-cell variability in ERK phosphorylation, rather than stochasticity in the ph..

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University of Melbourne Researchers

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