Journal article

Foxp3( ) Tregs from Langerhans cell histiocytosis lesions co-express CD56 and have a definitively regulatory capacity

Jenee Mitchell, Jason Kelly, Egle Kvedaraite, Tatiana von Bahr Greenwood, Jan-Inge Henter, Daniel G Pellicci, Stuart P Berzins, George Kannourakis

CLINICAL IMMUNOLOGY | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2020

Abstract

Langerhans cell histiocytosis (LCH) lesions contain myeloid lineage 'LCH' cells. Regulatory T cells (Tregs) are also enriched within lesions, although their role in LCH pathogenesis is unknown. LCH cells are thought to produce the transforming growth factor beta (TGF-β) within lesions, however whether Tregs contribute is unestablished. Using flow cytometry, we analyzed relative frequencies of live Tregs from LCH patients and identified CD56 expression and TGF-β production by lesion Tregs. While CD56+ Tregs were enriched in lesions, overall CD56+ T cells were reduced in the blood from active LCH patients compared to non-active disease patients, and there was a negative correlation between CD8..

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Grants

Funding Acknowledgements

We are thankful for the participation of patients and healthy donors in this study. A portion of LCH patient peripheral blood samples and coded data were supplied by the Children's Cancer Centre Tissue Bank at the Murdoch Children's Research Institute and The Royal Children's Hospital (www.mcri.edu.au/childrenscancercentretissuebank).Establishment and running of the Children's Cancer Centre Tissue Bank is made possible through generous support by Cancer In Kids@RCH(www.cika.org.au), Leukaemia Auxiliary at RCH (LARCH), the Murdoch Children's Research Institute and The Royal Children's Hospital Foundation.This work was supported by grants from the Swedish Children's Cancer Foundation and the Swedish Cancer Foundation. J.M. was supported by an Australian Government Research Training Program (RTP) Stipend and RTP Fee-Offset Scholarship through Federation University Australia.