A compensatory mutagenesis study of a conserved hairpin in the M gene segment of influenza A virus shows its role in virus replication
MI Spronken, CE van de Sandt, EP de Jongh, O Vuong, S van der Vliet, TM Bestebroer, RCL Olsthoorn, GF Rimmelzwaan, RAM Fouchier, AP Gultyaev
RNA Biology | TAYLOR & FRANCIS INC | Published : 2017
RNA structures are increasingly recognized to be of importance during influenza A virus replication. Here, we investigated a predicted conserved hairpin in the M gene segment (nt 967-994) within the region of the vRNA 5' packaging signal. The existence of this RNA structure and its possible role in virus replication was investigated using a compensatory mutagenesis approach. Mutations were introduced in the hairpin stem, based on natural variation. Virus replication properties were studied for the mutant viruses with disrupted and restored RNA structures. Viruses with structure-disrupting mutations had lower virus titers and a significantly reduced median plaque size when compared with the w..View full abstract
Awarded by NIAID/NIH
This work was financially supported by NIAID/NIH contract HSN272201400008C.