Journal article
Application of an indirect MilA ELISA for the detection of Mycoplasma bovis antibodies in bovine milk
AAB Al-Farha, N Wawegama, F Hemmatzadeh, S Firestone, J Moffat, GA Kojouri, AA Azari, R Amanollahi, A Hoare, K Petrovski
Turkish Journal of Veterinary and Animal Sciences | Tubitak Scientific & Technological Research Council Turkey | Published : 2020
DOI: 10.3906/vet-1811-62
Abstract
The objective of this study was to detect Mycoplasma bovis specific antibodies using a recently reported MilA ELISA with the aim to detect M. bovis antibodies in milk. An indirect ELISA, based on a recombinant fragment of the Mycoplasma immunogenic lipase A (MilA) protein, was conducted on 291 milk samples for the detection of M. bovis antibodies. Samples were also tested with conventional Mycoplasma culture and M. bovis PCR. Samples were collected from individual cows from 2 commercial dairy herds in South Australia. Of 291 samples tested, 68 (23.4%) were detected positive for M. bovis antibodies, 150 (51.5%) were positive for M. bovis in PCR and 166 (57.0%) in bacterial culture. These resu..
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Funding Acknowledgements
The authors would like to express their special thanks to the farmer and farm staff for their cooperation and interest in the study. Abd Al-Bar Al-Farha was partially funded by the Higher Committee for Education Development in Iraq in the form of a scholarship. All field and laboratory procedures were financially supported by the University of Adelaide.