Journal article

Further development of a reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of foot-and-mouth disease virus and validation in the field with use of an internal positive control

Carolyn Bath, Megan Scott, Puspa Maya Sharma, Ratna B Gurung, Yoenten Phuentshok, Stephen Pefanis, Axel Colling, Nagendrakumar Singanallur Balasubramanian, Simon M Firestone, Sahawatchara Ungvanijban, Jadsada Ratthanophart, John Allen, Grant Rawlin, Mark Fegan, Brendan Rodoni

Transboundary and Emerging Diseases | WILEY | Published : 2020

Abstract

Foot-and-mouth disease (FMD) is a highly contagious viral disease of cloven-hooved animals. Global outbreaks have highlighted the significant economic, trade, psychosocial and animal welfare impacts that can arise from the detection of disease in previously 'FMD-free' countries. Rapid and early diagnosis provides significant advantages in disease control and minimization of deleterious consequences. We describe the process of further development and validation of a reverse-transcription loop-mediated isothermal amplification foot-and-mouth disease virus (RT-LAMP-FMDV) test, using a published LAMP primer set, for use in the field. An internal positive control (IPC) was designed and introduced..

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University of Melbourne Researchers