Global Trends in Proteome Remodeling of the Outer Membrane Modulate Antimicrobial Permeability in Klebsiella pneumoniae
Andrea Rocker, Jake A Lacey, Matthew J Belousoff, Jonathan J Wilkscha, Richard A Strugnell, Mark R Davies, Trevor Lithgow
MBIO | AMER SOC MICROBIOLOGY | Published : 2020
In Gram-negative bacteria, the permeability of the outer membrane governs rates of antibiotic uptake and thus the efficacy of antimicrobial treatment. Hydrophilic drugs like β-lactam antibiotics depend on diffusion through pore-forming outer membrane proteins to reach their intracellular targets. In this study, we investigated the distribution of porin genes in more than 2,700 Klebsiella isolates and found a widespread loss of OmpK35 functionality, particularly in those strains isolated from clinical environments. Using a defined set of outer-membrane-remodeled mutants, the major porin OmpK35 was shown to be largely responsible for β-lactam permeation. Sequence similarity network analysis ch..View full abstract
Awarded by National Health and Medical Research Council of Australia
The research was supported by program grant 1092262 from the National Health and Medical Research Council of Australia.