Journal article

Structural dynamics in proteins induced by and probed with X-ray free-electron laser pulses

Karol Nass, Alexander Gorel, Malik M Abdullah, Andrew Martin, Marco Kloos, Agostino Marinelli, Andrew Aquila, Thomas RM Barends, Franz-Josef Decker, R Bruce Doak, Lutz Foucar, Elisabeth Hartmann, Mario Hilpert, Mark S Hunter, Zoltan Jurek, Jason E Koglin, Alexander Kozlov, Alberto A Lutman, Gabriela Nass Kovacs, Christopher M Roome Show all

Nature Communications | NATURE PUBLISHING GROUP | Published : 2020

Abstract

X-ray free-electron lasers (XFELs) enable crystallographic structure determination beyond the limitations imposed upon synchrotron measurements by radiation damage. The need for very short XFEL pulses is relieved through gating of Bragg diffraction by loss of crystalline order as damage progresses, but not if ionization events are spatially non-uniform due to underlying elemental distributions, as in biological samples. Indeed, correlated movements of iron and sulfur ions were observed in XFEL-irradiated ferredoxin microcrystals using unusually long pulses of 80 fs. Here, we report a femtosecond time-resolved X-ray pump/X-ray probe experiment on protein nanocrystals. We observe changes in th..

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Grants

Awarded by Cluster of Excellence 'Advanced Imaging of Matter' of the Deutsche Forschungsgemeinschaft (DFG)


Awarded by U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences


Awarded by National Institutes of Health


Funding Acknowledgements

This research was supported by the Max Planck Society. The work performed by the Hamburg group was supported in part by the Cluster of Excellence 'Advanced Imaging of Matter' of the Deutsche Forschungsgemeinschaft (DFG)-EXC 2056-project ID 390715994. We thank Dr. Roland van Gessel, Bracco Imaging Deutschland, Konstanz, Germany, for the very generous gift of the sample of gadoteridol and Beatrice Latz for support making capillaries. Use of the Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, is supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences under Contract No. DE-AC02-76SF00515. Parts of the sample injector used at LCLS for this research was funded by the National Institutes of Health, P41GM103393, formerly P41RR001209.