Journal article

Novel additive for sperm cryopreservation media: Holotheria parva coelomic cavity extract protects human spermatozoa against oxidative stress-A pilot study

Zahra Khashavi, Ahmad Homaei, Fahimeh Koohnavard, Ehsan Kamrani, Marcella Spinaci, Rodney B Luwor, Mahsa Archang, Ashok Agarwal, Ralf Henkel

ANDROLOGIA | WILEY | Published : 2020

Abstract

Cryopreservation is the most effective method for preserving semen for a long period of time. However, during the freeze-thaw process, production of reactive oxygen species (ROS) leads to a steep reduction in sperm fertility indices. In this study, we tested the effects of the extract of the coelomic cavity of five Holotheria parva, a marine organism rich in antioxidants, for its ROS-scavenging activity and cryoprotective effects on oxidative stress. Using a total of 50 semen samples, our results demonstrated that doses of 250 and 500 µg/ml of H. parva coelomic cavity extract significantly increased sperm vitality as compared to the control (p < .05). The addition of 250 µg/ml of the extract..

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