Journal article

HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV

Daniella H Hock, Boris Reljic, Ching-Seng Ang, Linden Muellner-Wong, Hayley S Mountford, Alison G Compton, Michael T Ryan, David R Thorburn, David A Stroud

Molecular and Cellular Proteomics | American Society for Biochemistry and Molecular Biology | Published : 2020


Assembly factors play a critical role in the biogenesis of mitochondrial respiratory chain complexes I-IV where they assist in the membrane insertion of subunits, attachment of co-factors, and stabilization of assembly intermediates. The major fraction of complexes I, III and IV are present together in large molecular structures known as respiratory chain supercomplexes. Several assembly factors have been proposed as required for supercomplex assembly, including the hypoxia inducible gene 1 domain family member HIGD2A. Using gene-edited human cell lines and extensive steady state, translation and affinity enrichment proteomics techniques we show that loss of HIGD2A leads to defects in the de..

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Awarded by National Health and Medical Research Council (NHMRC)

Awarded by NHMRC Biomedical Postgraduate Scholarship

Funding Acknowledgements

We acknowledge funding from the National Health and Medical Research Council (NHMRC Project Grants 1125390, 1140906 to D.A.S. and M.T.R. and 1068409 to D.R.T.; NHMRC Fellowships 1140851 to D.A.S. and 1155244 to D.R.T.). D.H.H. is supported by a Melbourne International Research Scholarship and Mito Foundation Top-up Scholarship, and H.S.M. by an NHMRC Biomedical Postgraduate Scholarship (1017174). We acknowledge support from the Victorian Government's Operational Infrastructure Support Program.