Host CD8 alpha( ) and CD103( ) dendritic cells prime transplant antigen-specific CD8( ) T cells via cross-dressing
Bin Li, Chunni Lu, Sara Oveissi, Jing Song, Kun Xiao, Damien Zanker, Mubin Duan, Jianxin Chen, Huji Xu, Quanming Zou, Chao Wu, Jonathan W Yewdell, Weisan Chen
Immunology & Cell Biology | WILEY | Published : 2020
The participation of dendritic cells (DCs) in CD8+ T-cell-mediated allograft rejection is a long-standing question of great clinical relevance for tissue transplantation. Here, we show that Batf3-/- mice demonstrate delayed allo-skin graft rejection and are deficient in priming allo-specific CD8+ T cells. Batf3-/- mouse priming is restored by transferring either purified CD8α+ or CD103+ DCs, demonstrating the critical role of these cells in alloreactivity. Using Db -restricted antiviral F5 transgenic T-cell receptor T cells, which we demonstrate are alloreactive with H-2Kd , we show that cross-dressing of CD8α+ and CD103+ primes CD8+ T-cell or allo-specific responses in vitro and in vivo. Th..View full abstract
Awarded by NHMRC program
This project was partly supported by the NHMRC program grant 567122 to WC. BL was supported by a scholarship from China Scholarship Council. JWY is supported by the DIR, NIAID, NIH.