Journal article

Open database searching enables the identification and comparison of bacterial glycoproteomes without defining glycan compositions prior to searching

Ameera Raudah Ahmad Izaham, Nichollas Scott

Cold Spring Harbor Laboratory | Published : 2020


ABSTRACT Mass spectrometry has become an indispensable tool for the characterisation of glycosylation across biological systems. Our ability to generate rich fragmentation of glycopeptides has dramatically improved over the last decade yet our informatic approaches still lag behind. While glycoproteomic informatics approaches using glycan databases have attracted considerable attention, database independent approaches have not. This has significantly limited high throughput studies of unusual or atypical glycosylation events such as those observed in bacteria. As such, computational approaches to examine bacterial glycosylation and identify chemically diverse glycans are desperately needed. ..

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University of Melbourne Researchers