The Impact of MHC Class I Dose on Development and Maintenance of the Polyclonal Naive CD8( ) T Cell Repertoire
Xavier YX Sng, Jasmine Li, Pirooz Zareie, Lisa M Assmus, Jason KC Lee, Claerwen M Jones, Stephen J Turner, Stephen R Daley, Kylie M Quinn, Nicole L La Gruta
JOURNAL OF IMMUNOLOGY | AMER ASSOC IMMUNOLOGISTS | Published : 2020
Naive CD8+ T cell survival in the periphery is critically dependent on tonic TCR signaling through peptide + MHC class I (MHCI) recognition; however, little is known about how natural variation in MHCI levels impacts the naive CD8+ T cell repertoire. Using mice that are hemizygous or homozygous for a single MHCI allele, we showed that despite a reduction in peripheral CD8+ T cell numbers of ∼50% in MHCI hemizygous mice, MHCI levels had no notable impact on the rate of thymic generation or emigration of CD8 single-positive T cells. Moreover, the peripheral T cell repertoire in hemizygous mice showed selective retention of T cell clonotypes with a greater competitive advantage as evidenced by ..View full abstract
Awarded by Australian Research Council
Awarded by National Health and Medical Research Council (NHMRC)
This work was supported by a Sylvia and Charles Viertel Charitable Foundation Senior Medical Research Fellowship, Australian Research Council Future Fellowship FT170100174, National Health and Medical Research Council (NHMRC) Program Grant APP1071916 (to N.L.L.G. and S.J.T.), an NHMRC Principal Research Fellowship (to S.J.T.), and a Monash University Biomedicine Discovery Scholarship (to X.Y.X.S.).