Restricted Sequence Variation in Streptococcus pyogenes Penicillin Binding Proteins
Andrew Hayes, Jake A Lacey, Jacqueline M Morris, Mark R Davies, Steven YC Tong
mSphere | AMER SOC MICROBIOLOGY | Published : 2020
A recent clinical report has linked Streptococcus pyogenes β-lactam antibiotic resistance to mutation in the penicillin binding protein (PBP) PBP2x. To determine whether this is an isolated case or reflects a broader prevalence of mutations that might confer reduced β-lactam susceptibility, we investigated the relative frequency of PBP sequence variation within a global database of 9,667 S. pyogenes isolates. We found that mutations in S. pyogenes PBPs (PBP2x, PBP1a, PBP1b, and PBP2a) occur infrequently across this global database, with fewer than 3 amino acid changes differing between >99% of the global population. Only 4 of the 9,667 strains contained mutations near transpeptidase active s..View full abstract
Awarded by Australian National Health and Medical Research Council (NHMRC)
Awarded by NHMRC
This work was supported by Australian National Health and Medical Research Council (NHMRC) project grant number 1098319. S.Y.C.T. is an NHMRC Career Development Fellow (number 1145033). M.R.D. is the recipient of a University of Melbourne C. R. Roper Fellowship.