Journal article

Restricted Sequence Variation in Streptococcus pyogenes Penicillin Binding Proteins

Andrew Hayes, Jake A Lacey, Jacqueline M Morris, Mark R Davies, Steven YC Tong



A recent clinical report has linked Streptococcus pyogenes β-lactam antibiotic resistance to mutation in the penicillin binding protein (PBP) PBP2x. To determine whether this is an isolated case or reflects a broader prevalence of mutations that might confer reduced β-lactam susceptibility, we investigated the relative frequency of PBP sequence variation within a global database of 9,667 S. pyogenes isolates. We found that mutations in S. pyogenes PBPs (PBP2x, PBP1a, PBP1b, and PBP2a) occur infrequently across this global database, with fewer than 3 amino acid changes differing between >99% of the global population. Only 4 of the 9,667 strains contained mutations near transpeptidase active s..

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Awarded by Australian National Health and Medical Research Council (NHMRC)

Awarded by NHMRC

Awarded by National Health and Medical Research Council of Australia

Funding Acknowledgements

This work was supported by Australian National Health and Medical Research Council (NHMRC) project grant number 1098319. S.Y.C.T. is an NHMRC Career Development Fellow (number 1145033). M.R.D. is the recipient of a University of Melbourne C. R. Roper Fellowship.