Journal article

In vivo multiplex molecular imaging of vascular inflammation using surface-enhanced Raman spectroscopy

Jonathan Noonan, Steven M Asiala, Gianluca Grassia, Neil MacRitchie, Kirsten Gracie, Jake Carson, Matthew Moores, Mark Girolami, Angela C Bradshaw, Tomasz J Guzik, Gavin R Meehan, Hannah E Scales, James M Brewer, Iain B McInnes, Naveed Sattar, Karen Faulds, Paul Garside, Duncan Graham, Pasquale Maffia

THERANOSTICS | IVYSPRING INT PUBL | Published : 2018

Abstract

Vascular immune-inflammatory responses play a crucial role in the progression and outcome of atherosclerosis. The ability to assess localized inflammation through detection of specific vascular inflammatory biomarkers would significantly improve cardiovascular risk assessment and management; however, no multi-parameter molecular imaging technologies have been established to date. Here, we report the targeted in vivo imaging of multiple vascular biomarkers using antibody-functionalized nanoparticles and surface-enhanced Raman scattering (SERS). Methods: A series of antibody-functionalized gold nanoprobes (BFNP) were designed containing unique Raman signals in order to detect intercellular adh..

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Grants

Awarded by Engineering and Physical Sciences Research Council (EPSRC)


Awarded by European Commission Marie Sklodowska-Curie Individual Fellowships


Awarded by British Heart Foundation


Awarded by Wellcome Trust


Funding Acknowledgements

This work was supported by the Engineering and Physical Sciences Research Council (EPSRC) grant EP/L014165/1, the European Commission Marie Sklodowska-Curie Individual Fellowships 661369, the British Heart Foundation grant RE/13/5/30177 and the Wellcome Trust grant WT095507MA.