Journal article
Hypertension and increased endothelial mechanical stretch promote monocyte differentiation and activation: Roles of STAT3, interleukin 6 and hydrogen peroxide
R Loperena, JP Van Beusecum, HA Itani, N Engel, F Laroumanie, L Xiao, F Elijovich, CL Laffer, JS Gnecco, J Noonan, P Maffia, B Jasiewicz-Honkisz, M Czesnikiewicz-Guzik, T Mikolajczyk, T Sliwa, S Dikalov, CM Weyand, TJ Guzik, DG Harrison
Cardiovascular Research | OXFORD UNIV PRESS | Published : 2018
DOI: 10.1093/cvr/cvy112
Abstract
Aims Monocytes play an important role in hypertension. Circulating monocytes in humans exist as classical, intermediate, and non-classical forms. Monocyte differentiation can be influenced by the endothelium, which in turn is activated in hypertension by mechanical stretch. We sought to examine the role of increased endothelial stretch and hypertension on monocyte phenotype and function. Methods Human monocytes were cultured with confluent human aortic endothelial cells undergoing either 5% or 10% cyclical and results stretch. We also characterized circulating monocytes in normotensive and hypertensive humans. In addition, we quantified accumulation of activated monocytes and monocyte-derive..
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Awarded by National Institutes of Health
Funding Acknowledgements
This work was supported by National Heart and Blood Institute of the National Institutes of Health Grants [R01HL039006-27, R01HL125865-04, and P01HL129941], an American Heart Association Strategically Focused Research Network Grant (14SFRN20420046) and National Heart, Lung, and Blood Institute of the National Institutes of Health, Ruth L. Kirschstein National Research Service Award (NRSA) Individual Predoctoral Fellowship to Promote Diversity in Health-Related Research (F31HL132526). The work performed in Glasgow was supported by the Engineering and Physical Sciences Research Council (EPSRC) grant EP/L014165/1 and the British Heart Foundation grant RE/13/5/30177. Dr Guzik is a recipient of the Wellcome Trust Senior Biomedical Fellowship, National Science Centre of Poland (No. 2011/03/B/NZ4/02454) and BHF Centre of Research Excellence (RE/13/5/30177).