Journal article

Hypertension and increased endothelial mechanical stretch promote monocyte differentiation and activation: roles of STAT3, interleukin 6 and hydrogen peroxide

Roxana Loperena, Justin P Van Beusecum, Hana A Itani, Noah Engel, Fanny Laroumanie, Liang Xiao, Fernando Elijovich, Cheryl L Laffer, Juan S Gnecco, Jonathan Noonan, Pasquale Maffia, Barbara Jasiewicz-Honkisz, Marta Czesnikiewicz-Guzik, Tomasz Mikolajczyk, Tomasz Sliwa, Sergey Dikalov, Cornelia M Weyand, Tomasz J Guzik, David G Harrison

CARDIOVASCULAR RESEARCH | OXFORD UNIV PRESS | Published : 2018

Abstract

Aims: Monocytes play an important role in hypertension. Circulating monocytes in humans exist as classical, intermediate, and non-classical forms. Monocyte differentiation can be influenced by the endothelium, which in turn is activated in hypertension by mechanical stretch. We sought to examine the role of increased endothelial stretch and hypertension on monocyte phenotype and function. Methods and results: Human monocytes were cultured with confluent human aortic endothelial cells undergoing either 5% or 10% cyclical stretch. We also characterized circulating monocytes in normotensive and hypertensive humans. In addition, we quantified accumulation of activated monocytes and monocyte-deri..

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Grants

Awarded by National Heart and Blood Institute of the National Institutes of Health Grants


Awarded by American Heart Association Strategically Focused Research Network Grant


Awarded by Ruth L. Kirschstein National Research Service Award (NRSA) Individual Predoctoral Fellowship


Awarded by Engineering and Physical Sciences Research Council (EPSRC)


Awarded by British Heart Foundation


Awarded by National Science Centre of Poland


Awarded by BHF Centre of Research Excellence


Awarded by NATIONAL HEART, LUNG, AND BLOOD INSTITUTE


Awarded by NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES


Funding Acknowledgements

This work was supported by National Heart and Blood Institute of the National Institutes of Health Grants [R01HL039006-27, R01HL125865-04, and P01HL129941], an American Heart Association Strategically Focused Research Network Grant (14SFRN20420046) and National Heart, Lung, and Blood Institute of the National Institutes of Health, Ruth L. Kirschstein National Research Service Award (NRSA) Individual Predoctoral Fellowship to Promote Diversity in Health-Related Research (F31HL132526). The work performed in Glasgow was supported by the Engineering and Physical Sciences Research Council (EPSRC) grant EP/L014165/1 and the British Heart Foundation grant RE/13/5/30177. Dr Guzik is a recipient of the Wellcome Trust Senior Biomedical Fellowship, National Science Centre of Poland (No. 2011/03/B/NZ4/02454) and BHF Centre of Research Excellence (RE/13/5/30177).