Journal article

A Novel Triple-Cell Two-Dimensional Model to Study Immune-Vascular Interplay in Atherosclerosis

Jonathan Noonan, Gianluca Grassia, Neil MacRitchie, Paul Garside, Tomasz J Guzik, Angela C Bradshaw, Pasquale Maffia

FRONTIERS IN IMMUNOLOGY | FRONTIERS MEDIA SA | Published : 2019

Abstract

Atherosclerosis is a complex inflammatory pathology underpinning cardiovascular diseases (CVD), which are the leading cause of death worldwide. The interplay between vascular stromal cells and immune cells is fundamental to the progression and outcome of atherosclerotic disease, however, the majority of in vitro studies do not consider the implications of these interactions and predominantly use mono-culture approaches. Here we present a simple and robust methodology involving the co-culture of vascular endothelial (ECs) and smooth muscle cells (SMCs) alongside an inflammatory compartment, in our study containing THP-1 macrophages, for studying these complex interactions. Using this approach..

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Grants

Awarded by British Heart Foundation


Awarded by Engineering and Physical Sciences Research Council (EPSRC)


Awarded by European Commission Marie Sklodowska-Curie Individual Fellowships


Funding Acknowledgements

This work was supported by the British Heart Foundation grants PG/12/81/29897 and RE/13/5/30177; the Engineering and Physical Sciences Research Council (EPSRC) grant EP/L014165/1 and the European Commission Marie Sklodowska-Curie Individual Fellowships 661369.