Development of a sers-nanoparticle based system for the multiplex detection of vascular adhesion molecules

Abstract

Surface-enhanced Raman spectroscopy (SERS) is an emerging molecular imaging technique suitable for the highly sensitive simultaneous detection of multiple analytes. We aimed to apply SERS for the multiplex detection of adhesion molecules in human umbilical vein endothelial cells and macrophages in vitro, as a step towards applying this technology for simultaneously detecting multiple biomarkers of vascular inflammation and atherosclerotic plaque instability in vivo. Immunofluorescent microscopy and flow cytometry identified the expression of intercellular adhesion molecule (ICAM)−1, vascular cell adhesion molecule (VCAM)−1 and p-Selectin on human umbilical vein endothelial cells (HUVECs) fol..