Monocyte apoptotic bodies are vehicles for influenza A virus propagation
Georgia K Atkin-Smith, Mubing Duan, Damien J Zanker, Liyen Loh, Thi HO Nguyen, Marios Koutsakos, Tien Nguyen, Xiangrui Jiang, Julio Carrera, Thanh Kha Phan, Chuanxin Liu, Stephanie Paone, Sara Oveissi, Amy L Hodge, Amy A Baxter, Katherine Kedzierska, Jason M Mackenzie, Mark D Hulett, Pamuk Bilsel, Weisan Chen Show all
COMMUNICATIONS BIOLOGY | NATURE PUBLISHING GROUP | Published : 2020
The disassembly of apoptotic cells into small membrane-bound vesicles termed apoptotic bodies (ApoBDs) is a hallmark of apoptosis; however, the functional significance of this process is not well defined. We recently discovered a new membrane protrusion (termed beaded apoptopodia) generated by apoptotic monocytes which fragments to release an abundance of ApoBDs. To investigate the function of apoptotic monocyte disassembly, we used influenza A virus (IAV) infection as a proof-of-concept model, as IAV commonly infects monocytes in physiological settings. We show that ApoBDs generated from IAV-infected monocytes contained IAV mRNA, protein and virions and consequently, could facilitate viral ..View full abstract
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Awarded by National Health and Medical Research Council of Australia
We thank Peter Lock and the LIMS BioImaging facility for assistance with microscopy, and members of the La Trobe Animal Research and Teaching Facility for assistance with animal work. This work has been supported by grants from the National Health and Medical Research Council of Australia (GNT1125033 and GNT1140187), La Trobe University Understanding Disease and the CASS Foundation to I.K.H.P., and grants from the National Health and Medical Research Council of Australia (GNT1071916) to W.C. and K.K. K.K. is an NHMRC senior research level B fellow (GNT1102792).