Journal article

The best type of inoculum for testing the antifungal drug susceptibility of Microsporum canis: In vivo and in vitro results

Chioma Inyang Aneke, Wafa Rhimi, Claudia Pellicoro, Cinzia Cantacessi, Domenico Otranto, Claudia Cafarchia

Mycoses | WILEY | Published : 2020

Abstract

BACKGROUND: Data correlating in vitro drug susceptibility of Microsporum canis with clinical outcomes of its infections are lacking as well as the most suitable inoculum and incubation time in broth microdilution assays. OBJECTIVES AND METHODS: Microsporum canis strains were collected from animal hosts that tested positive (Group I; n = 13) and negative (Group II; n = 14) to this pathogen following itraconazole (ITC) therapy. In vitro ITC susceptibility was assessed according to the Clinical Laboratory Standards Institute (CLSI M38-A2) methodology using conidia, hypha-conidia and arthroconidia at 3 and 7 days of incubation in order to assess the most suitable inoculum and incubation time. Su..

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University of Melbourne Researchers