Journal article

Interaction of Plasmodium falciparum casein kinase 1 with components of host cell protein trafficking machinery

Mitchell B Batty, Ralf B Schittenhelm, Dominique Dorin-Semblat, Christian Doerig, Jose F Garcia-Bustos

IUBMB LIFE | WILEY | Published : 2020

Abstract

A pool of Plasmodium falciparum casein kinase 1 (PfCK1) has been shown to localize to the host red blood cell (RBC) membrane and be secreted to the extracellular medium during trophozoite stage of development. We attempted to identify mechanisms for secretion of PfCK1 and its appearance on the RBC membrane. We found that two host proteins with established functions in membrane trafficking in higher eukaryotes, GTPase-activating protein and Vps9 domain-containing protein 1 (GAPVD1), and Sorting nexin 22, consistently co-purify with PfCK1, suggesting that the parasite utilizes trafficking pathways previously thought to be inactive in RBCs. Furthermore, reciprocal immunoprecipitation experiment..

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University of Melbourne Researchers