Journal article

Experimental Evolution In Vivo To Identify Selective Pressures during Pneumococcal Colonization

Vaughn S Cooper, Erin Honsa, Hannah Rowe, Christopher Deitrick, Amy R Iverson, Jonathan J Whittall, Stephanie L Neville, Christopher A McDevitt, Colin Kietzman, Jason W Rosch

MSYSTEMS | AMER SOC MICROBIOLOGY | Published : 2020

Abstract

Experimental evolution is a powerful technique to understand how populations evolve from selective pressures imparted by the surrounding environment. With the advancement of whole-population genomic sequencing, it is possible to identify and track multiple contending genotypes associated with adaptations to specific selective pressures. This approach has been used repeatedly with model species in vitro, but only rarely in vivo Herein we report results of replicate experimentally evolved populations of Streptococcus pneumoniae propagated by repeated murine nasal colonization with the aim of identifying gene products under strong selection as well as the population genetic dynamics of infectio..

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Grants

Awarded by Australian Research Council (ARC)



Funding Acknowledgements

J.W.R. and V.S.C. are supported by grant 1U01AI124302. J.W.R. is supported by grant 1R01AI110618. This work was supported by St. Jude Children's Hospital and ALSAC. This work was supported by the Australian Research Council (ARC) Discovery Project grant DP170102102 to J.W.R. and C.A.M. S.L.N. is a National Health and Medical Research Council Early Career Research Fellow (1142695), and C.A.M. is an ARC Future Fellow (FT170100006).