Liver sinusoidal endothelial cells promote the differentiation and survival of mouse vascularised hepatobiliary organoids
Kiryu K Yap, Yi-Wen Gerrand, Aaron M Dingle, George C Yeoh, Wayne A Morrison, Geraldine M Mitchell
BIOMATERIALS | ELSEVIER SCI LTD | Published : 2020
The structural and physiological complexity of currently available liver organoids is limited, thereby reducing their relevance for drug studies, disease modelling, and regenerative therapy. In this study we combined mouse liver progenitor cells (LPCs) with mouse liver sinusoidal endothelial cells (LSECs) to generate hepatobiliary organoids with liver-specific vasculature. Organoids consisting of 5x103 cells were created from either LPCs, or a 1:1 combination of LPC/LSECs. LPC organoids demonstrated mild hepatobiliary differentiation in vitro with minimal morphological change; in contrast LPC/LSEC organoids developed clusters of polygonal hepatocyte-like cells and biliary ducts over a 7 day ..View full abstract
The authors gratefully acknowledge funding from the Australian National Health & Medical Research Council (NHMRC), Stafford Fox Foundation, O'Brien Institute Foundation, St Vincent's Hospital Melbourne Research Endowment Fund, Australian Catholic University, Bioplatforms Australia, University of Melbourne Centre for Stem Cell Systems, and the Victorian State Government's Department of Business Innovation Operational Infrastructure Support Program. KKY has received an Australian Government Research Training Program Scholarship, and research scholarships from the NHMRC, Australian and New Zealand Hepatic, Pancreatic and Biliary Association, and St Vincent's Institute Foundation, Melbourne.