Journal article

Unique properties of a subset of human pluripotent stem cells with high capacity for self-renewal

Kevin X Lau, Elizabeth A Mason, Joshua Kie, David P De Souza, Joachim Kloehn, Dedreia Tull, Malcolm J McConville, Andrew Keniry, Tamara Beck, Marnie E Blewitt, Matthew E Ritchie, Shalin H Naik, Daniela Zalcenstein, Othmar Korn, Shian Su, Irene Gallego Romero, Catrina Spruce, Christopher L Baker, Tracy C McGarr, Christine A Wells Show all

NATURE COMMUNICATIONS | NATURE PUBLISHING GROUP | Published : 2020

Abstract

Archetypal human pluripotent stem cells (hPSC) are widely considered to be equivalent in developmental status to mouse epiblast stem cells, which correspond to pluripotent cells at a late post-implantation stage of embryogenesis. Heterogeneity within hPSC cultures complicates this interspecies comparison. Here we show that a subpopulation of archetypal hPSC enriched for high self-renewal capacity (ESR) has distinct properties relative to the bulk of the population, including a cell cycle with a very low G1 fraction and a metabolomic profile that reflects a combination of oxidative phosphorylation and glycolysis. ESR cells are pluripotent and capable of differentiation into primordial germ ce..

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Grants

Awarded by Australian Research Council Special Research Initiative in StemCell Sciences


Funding Acknowledgements

This study was supported by the Australian Research Council Special Research Initiative in StemCell Sciences (SR1101002), and Bioplatforms Australia, through funding from the Australian Government National Collaborative Research Infrastructure Strategy. M.J.M. is a Principal Research Fellow of the National Health and Medical Research Council. M.E.B. was supported by a Bellberry-Viertel Senior Medical Research Fellowship.