Journal article

Surface Modification of Spider Silk Particles to Direct Biomolecular Corona Formation.

Alessia CG Weiss, Heike M Herold, Sarah Lentz, Matthew Faria, Quinn A Besford, Ching-Seng Ang, Frank Caruso, Thomas Scheibel

ACS Applied Materials and Interfaces | American Chemical Society | Published : 2020

Abstract

In recent years, spider silk-based materials have attracted attention because of their biocompatibility, processability, and biodegradability. For their potential use in biomaterial applications, i.e., as drug delivery systems and implant coatings for tissue regeneration, it is vital to understand the interactions between the silk biomaterial surface and the biological environment. Like most polymeric carrier systems, spider silk material surfaces can adsorb proteins when in contact with blood, resulting in the formation of a biomolecular corona. Here, we assessed the effect of surface net charge of materials made of recombinant spider silk on the biomolecular corona composition. In-depth pr..

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Grants

Awarded by Australian Research Council (ARC) Centre of Excellence in Convergent Bio-Nano Science and Technology


Awarded by Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)


Awarded by NHMRC Senior Principal Research Fellowship


Funding Acknowledgements

This research was conducted and funded by the Australian Research Council (ARC) Centre of Excellence in Convergent Bio-Nano Science and Technology (project CE140100036) (F.C.) and the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) through SFB 840 subproject A8 (T.S). F.C. acknowledges the award of an NHMRC Senior Principal Research Fellowship (GNT1135806). A. C.G.W. acknowledges funding from The University of Melbourne through Melbourne International Research Scholarships. A.C.G.W., H.M.H., S.L., F.C. and T.S. acknowledge funding from the German Academic Exchange Service (DAAD) through its thematic network Bayreuth-Melbourne Colloid/Polymer Network sponsored from funds of the Federal Ministry of Education and Research (BMBF). We thank Markus Rottmar and Eike Muller for help with collecting blood samples and further thank Vanessa Neubauer for fermentation and purification of the protein eADF4(C16)E<INF>8</INF>G.