Journal article

Effect of structural stability on endolysosomal degradation and T-cell reactivity of major shrimp allergen tropomyosin

Sandip D Kamath, Sandra Scheiblhofer, Christopher M Johnson, Yoan Machado, Thomas McLean, Aya C Taki, Paul A Ramsland, Swati Iyer, Isabella Joubert, Heidi Hofer, Michael Wallner, Josef Thalhamer, Jennifer Rolland, Robyn O'Hehir, Peter Briza, Fatima Ferreira, Richard Weiss, Andreas L Lopata

Allergy | WILEY | Published : 2020

Abstract

BACKGROUND: Tropomyosins are highly conserved proteins, an attribute that forms the molecular basis for their IgE antibody cross-reactivity. Despite sequence similarities, their allergenicity varies greatly between ingested and inhaled invertebrate sources. In this study, we investigated the relationship between the structural stability of different tropomyosins, their endolysosomal degradation patterns and T-cell reactivity. METHODS: We investigated the differences between four tropomyosins - the major shrimp allergen Pen m 1 and the minor allergens Der p 10 (dust mite), Bla g 7 (cockroach) and Ani s 3 (fish parasite) - in terms of IgE binding, structural stability, endolysosomal degradatio..

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Grants

Awarded by National Health and Medical Research Council


Awarded by Austrian Science Fund (FWF)


Funding Acknowledgements

National Health and Medical Research Council, Grant/Award Number: GNT1086656 and GNT1124143; Austrian Science Fund (FWF), Grant/Award Number: P26997_B13; Allergy and Immunology Foundation of Australasia (AIFA)