Journal article

A Sulfoglycolytic Entner-Doudoroff Pathway in Rhizobium leguminosarum bv. trifolii SRDI565

Jinling Li, Ruwan Epa, Nichollas E Scott, Dominik Skoneczny, Mahima Sharma, Alexander JD Snow, James P Lingford, Ethan D Goddard-Borger, Gideon J Davies, Malcolm J McConville, Spencer J Williams

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | AMER SOC MICROBIOLOGY | Published : 2020

Abstract

Rhizobia are nitrogen-fixing bacteria that engage in symbiotic relationships with plant hosts but can also persist as free-living bacteria in the soil and rhizosphere. Here, we show that free-living Rhizobium leguminosarum SRDI565 can grow on the sulfosugar sulfoquinovose (SQ) or the related glycoside SQ-glycerol using a sulfoglycolytic Entner-Doudoroff (sulfo-ED) pathway, resulting in production of sulfolactate (SL) as the major metabolic end product. Comparative proteomics supports the involvement of a sulfo-ED operon encoding an ABC transporter, sulfo-ED enzymes, and an SL exporter. Consistent with an oligotrophic lifestyle, proteomics data revealed little change in expression of the sulf..

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Grants

Awarded by Australian Research Council


Awarded by National Health and Medical Research Council of Australia


Funding Acknowledgements

This work was supported by grants from the Australian Research Council (DP180101957), the National Health and Medical Research Council of Australia (APP1100164 and GNT1139549), and the Leverhulme Trust; support from The Walter and Eliza Hall Institute of Medical Research, the Australian Cancer Research Fund; and a Victorian State Government Operational Infrastructure support grant. M.J.M. is an NHMRC Principal Research Fellow; G.J.D. is a Royal Society Ken Murray Research Fellow. J.L. is supported by a Ph.D. scholarship from the China Scholarship Council.