Journal article

High-fat diet selectively decreases bone marrow lin(-)/CD117( ) cell population in aging mice through increased ROS production

Yichao Xiao, Qingyi Zhu, Xuanyou Liu, Meng Jiang, Hong Hao, Hua Zhu, Peter J Cowan, Xiaoming He, Qiming Liu, Shenghua Zhou, Zhenguo Liu

Journal of Tissue Engineering and Regenerative Medicine | WILEY | Published : 2020

Abstract

Bone marrow (BM) stem cells (BMSCs) are an important source for cell therapy. The outcome of cell therapy could be ultimately associated with the number and function of donor BMSCs. The present study was to evaluate the effect of long-term high-fat diet (HFD) on the population of BMSCs and the role of reactive oxygen species (ROS) in aging mice. Forty-week-old male C57BL/6 mice were fed with HFD for 3 months with regular diet as control. Experiments were repeated when ROS production was reduced in mice treated with N-acetylcysteine (NAC) or using mice overexpressing antioxidant enzyme network (AON) of superoxide dismutase (SOD)1, SOD3, and glutathione peroxidase. BM and blood cells were anal..

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