Sequencing B cell receptors from ferrets (Mustela putorius furo)
Julius Wong, Celeste M Tai, Aeron C Hurt, Hyon-Xhi Tan, Stephen J Kent, Adam K Wheatley
PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2020
The domestic ferret (Mustela putorius furo) provides a critical animal model to study human respiratory diseases. However immunological insights are restricted due to a lack of ferret-specific reagents and limited genetic information about ferret B and T cell receptors. Here, variable, diversity and joining genes within the ferret kappa, lambda and heavy chain immunoglobulin loci were annotated using available genomic information. A multiplex PCR approach was derived that facilitated the recovery of paired heavy and light chain immunoglobulin sequences from single sorted ferret B cells, allowing validation of predicted germline gene sequences and the identification of putative novel germline..View full abstract
Awarded by NHMRC
The Melbourne WHO Collaborating Centre for Reference and Research on Influenza is supported by the Australian Government Department of Health. This work was supported by NHMRC programme grant #1052979 (SJK) and NHMRC project grant #1129099 (AKW). JW is supported by a Melbourne International Research Scholarship and Melbourne International Fee Remission Scholarship.