Journal article

Innate and Adaptive Anti-SIV Responses in Macaque Semen: Implications for Infectivity and Risk of Transmission

Karunasinee Suphaphiphat, Sibylle Bernard-Stoecklin, Celine Gommet, Benoit Delache, Nathalie Dereuddre-Bosquet, Stephen J Kent, Bruce D Wines, P Mark Hogarth, Roger Le Grand, Mariangela Cavarelli

FRONTIERS IN IMMUNOLOGY | FRONTIERS MEDIA SA | Published : 2020

Abstract

HIV-1 infection is transmitted primarily by sexual exposure, with semen being the principal contaminated fluid. However, HIV-specific immune response in semen has been understudied. We investigated specific parameters of the innate, cellular, and humoral immune response that may affect semen infectivity in macaques infected with SIVmac251. Serial semen levels of cytokines and chemokines, SIV-specific antibodies, neutralization, and FcγR-mediated functions and SIV-specific T-cell responses were assessed and compared to systemic responses across 53 cynomolgus macaques. SIV infection induced an overall inflammatory state in the semen. Several pro-inflammatory molecules correlated with SIV virus..

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Grants

Awarded by Sidaction Fonds de dotation Pierre Berge


Awarded by EU CHAARM project


Awarded by ANRS


Awarded by Franco-Thai Program


Awarded by EAVI2020 European project of the H2020 framework


Awarded by Marie Curie Individual fellowship


Awarded by Sidaction Fond de dotation Pierre Berge


Awarded by ANR


Funding Acknowledgements

This work was supported by the Programme Investissements d'Avenir (PIA), managed by the ANR under reference ANR-11-INBS-0008, funding the Infectious Disease Models and Innovative Therapies (IDMIT, Fontenay-aux-Roses, France) infrastructure, and ANR-10-EQPX-02-01, funding the FlowCyTech facility (IDMIT, Fontenay-aux-Roses, France). Studies were supported by the ANRS (n.14415) research program and the Sidaction Fond de dotation Pierre Berge 17-2AEQ-11545. Part of this program was also funded by EAVI2020 (n. 681137) European project of the H2020 framework. MC was a beneficiary of a Marie Curie Individual fellowship (658277). KS was supported by fellowships from the ANRS (n.2016-313) and from the Franco-Thai Program (n. 849249B). SB-S was supported by fellowships from EU CHAARM project (242135). CG was supported by fellowships from Sidaction Fonds de dotation Pierre Berge (N. AP-FPB-2011-03).